《植物生理学报》 2016, 52(10): 1491-1497

非洲冰草快速繁殖及试管苗玻璃化的影响因子

陈思¹, 张君¹, 黄洲¹, 饶微微¹, 薛施施¹, 庞基良^1,2,*

¹杭州师范大学生命与环境科学学院, 杭州310036; ²浙江省药用植物种质改良与质量控制技术重点实验室, 杭州310036

通信作者：庞基良；E-mail: pangrenshuiliang@aliyun.com

摘　要：

以非洲冰草顶芽为外植体, 建立了非洲冰草的组培快繁体系。结果表明: 非洲冰草种子适宜的消毒方法为70%酒精浸泡30 s后, 用0.1% HgCl₂ 消毒1 min; 种子在含有4 mmol•L^-1 KNO₃ 的育苗培养基上萌发率可达98%, 且幼苗长势最好; 培养基中添加低浓度的NaCl (40~120 mmol•L^-1)能有效降低试管苗玻璃化的发生; 幼苗在MS+0.5 mg•L^-1 6-BA+80 mmol•L^-1 NaCl+30 g•L^-1蔗糖+6 g•L^-1琼脂的培养基上玻璃化率仅为10.83%, 增殖系数可达2.76; 在不添加外源激素的生根培养基上均能获得健壮的生根苗, 生根率达100%; 幼苗经炼苗后进行移栽, 成活率可达71%。

关键词：非洲冰草; 快速繁殖; 玻璃化

收稿：2016-07-28   修定：2016-09-23

资助：杭州市科技计划(20150932H01)。

Rapid propagation and factors of vitrification in vitro of Mesembryanthemum crystallinum

CHEN Si¹, ZHANG Jun¹, HUANG Zhou¹, RAO Wei-Wei¹, XUE Shi-Shi¹, PANG Ji-Liang^{1,2 *}

¹School of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China; ²Zhejiang Province Key Laboratory of Medicinal Plant Germplasm Imp rovement and Quality Control Technology, Hangzhou 310036, China

Corresponding author: PANG Ji-Liang; E-mail: pangrenshuiliang@aliyun.com

Abstract:

An efficient plant regeneration procedure has been established from terminal bud explants of the common ice plant, Mesembryanthemum crystallinum. The best protocol for seed sterilization was that the seeds were immersed in 70% alcohol for 30 seconds, then were disinfected by dipping into 0.1% HgCl₂ for 1 min. The optimum medium of growing seedlings was MS medium supplemented with 4 mmol•L^-1 KNO₃. In this medium, the seedling germination rate was up to 98%, and the seedlings grew best. Medium supplemented with 40–120 mmol•L^-1 NaCl could effectively reduce the occurrence of vitrification. The suitable proliferation medium was MS medium supplemented with 0.5 mg•L^-1 6-BA, 80 mmol•L^-1 NaCl, 30 g•L^-1 sucrose and 6 g•L^-1 agar, on which the terminal bud’s vitrification rate was only 10.83% and proliferation coefficient reached 2.76. Without growth regulators, regenerated plants rooted on MS medium with 100% efficiency. Mature, regenerated plants were fertile and the survival rate could reach 71%.

Key words: Mesembryanthemum crystallinum; rapid propagation; vitrification